pericyte growth media  (PromoCell)

Journal: Scientific Reports

Article Title: The regulation of placental pericyte function through transforming growth factor β -1 signalling

doi: 10.1038/s41598-025-20432-9

Figure Lengend Snippet: TGF β -1 signalling via canonical receptor ALK5 in placental pericytes. ( a ) Representative image of ALK5/TGF β R1 expression (ICC/IF; green), WGA plasma membrane staining (red), nuclear stain (blue). Scale bar 150 μm; ( b ) RT-PCR analysis of Type I ALK5 TGFBR1 (91 bp) and Type II receptor, TGFBR2 (99 bp) in placental pericytes (n = 3); ( c ) pSMAD2 (52kD) and GAPDH (37kD) Western Blot. Treatment groups (n = 3): VEH control (DMSO), TGF β -1 (10 ng/mL), TGF β -1 + ALK5 in. (10 ng/mL + 2 μM, respectively); ( d ) Western Blot densitometry analysis; one-way ANOVA. Stars indicate statistical significance between treatment groups, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).

Article Snippet: To assess pericyte phagocytosis , FACS-isolated primary human placental pericytes (PromoCell) were cultured in ready-to-use pericyte growth media (PromoCell), seeded at 3.0 × 10 3 cells/cm 2 and allowed to establish in 5% CO 2 in 37 °C humidified air for 48 h before TGF β -1 treatment.

Techniques: Expressing, Clinical Proteomics, Membrane, Staining, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control

Journal: Scientific Reports

Article Title: The regulation of placental pericyte function through transforming growth factor β -1 signalling

doi: 10.1038/s41598-025-20432-9

Figure Lengend Snippet: ELISA analysis of angiogenic factors secreted from placental pericytes. Treatment groups (n = 3): VEH control (DMSO), TGF β -1 (10 ng/mL), TGF β -1 + ALK5 in; ( a ) VEGF secretion (Kruskal–Wallis analysis); ( b ) MMP-2 secretion (one-way ANOVA). ( c ) ANG-1 secretion (one-way ANOVA). ELISA results normalized to cell number. Stars indicate statistical significance between treatment groups, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).

Article Snippet: To assess pericyte phagocytosis , FACS-isolated primary human placental pericytes (PromoCell) were cultured in ready-to-use pericyte growth media (PromoCell), seeded at 3.0 × 10 3 cells/cm 2 and allowed to establish in 5% CO 2 in 37 °C humidified air for 48 h before TGF β -1 treatment.

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Scientific Reports

Article Title: The regulation of placental pericyte function through transforming growth factor β -1 signalling

doi: 10.1038/s41598-025-20432-9

Figure Lengend Snippet: Collagen IV, fibronectin and laminin staining in placental pericytes. Representative ICC/IF images (VEH-treated placental pericytes); ( a ) Collagen IV (red), nuclear stain (blue); data expressed as area and mean fluorescent intensity (MFI); one-way ANOVA, normalized to cell number; ( b ) Fibronectin (red), nuclear stain (blue), data expressed as area and MFI; one-way ANOVA, normalized to cell number; ( c ) Laminin (red), nuclear stain (blue); data expressed as area; Kruskal–Wallis analysis and MFI; one-way ANOVA, normalized to cell number. Scale Bars = 150 μm. Stars indicate statistical significance between treatment groups, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).

Article Snippet: To assess pericyte phagocytosis , FACS-isolated primary human placental pericytes (PromoCell) were cultured in ready-to-use pericyte growth media (PromoCell), seeded at 3.0 × 10 3 cells/cm 2 and allowed to establish in 5% CO 2 in 37 °C humidified air for 48 h before TGF β -1 treatment.

Techniques: Staining

Journal: Scientific Reports

Article Title: The regulation of placental pericyte function through transforming growth factor β -1 signalling

doi: 10.1038/s41598-025-20432-9

Figure Lengend Snippet: Placental pericyte secretion of inflammatory factors. Analysis of inflammatory secretion (ELISA). Treatment groups: VEH control (DMSO), TGF β -1 (10 ng/mL), TGF β -1 + ALK5 in. (10 ng/mL + 2 μM, respectively); ( a ) IL-6 secretion; one-way ANOVA; ( b ) MCP-1 secretion; one-way ANOVA; ( c ) sVCAM-1 secretion; one-way ANOVA; ( d ) CX3CL1 secretion; one-way ANOVA. ( e ) IL-8 secretion; Kruskal–Wallis analysis. Data (n = 3); stars indicate statistical significance between treatment groups ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Error bars (SEM).

Article Snippet: To assess pericyte phagocytosis , FACS-isolated primary human placental pericytes (PromoCell) were cultured in ready-to-use pericyte growth media (PromoCell), seeded at 3.0 × 10 3 cells/cm 2 and allowed to establish in 5% CO 2 in 37 °C humidified air for 48 h before TGF β -1 treatment.

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Scientific Reports

Article Title: The regulation of placental pericyte function through transforming growth factor β -1 signalling

doi: 10.1038/s41598-025-20432-9

Figure Lengend Snippet: Alternate TGF β -1 signalling pathway in placental pericytes: Type I receptor ACVRL1 (ALK1, 94 bp) expression in placental pericytes, as assessed by RT-PCR; original image shown.

Article Snippet: To assess pericyte phagocytosis , FACS-isolated primary human placental pericytes (PromoCell) were cultured in ready-to-use pericyte growth media (PromoCell), seeded at 3.0 × 10 3 cells/cm 2 and allowed to establish in 5% CO 2 in 37 °C humidified air for 48 h before TGF β -1 treatment.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: Scientific Reports

Article Title: The regulation of placental pericyte function through transforming growth factor β -1 signalling

doi: 10.1038/s41598-025-20432-9

Figure Lengend Snippet: Phagocytosis capacity of placental pericytes. Treatment groups: VEH control and TGF β -1 (10 ng/mL) with and without FluoSphere incubation; ( a ) Representative flow cytometry histograms to identify the percentage of the population with and without phagocytosed FluoSpheres based on fluorescence; ( b ) Analysis of phagocytosis (Mann–Whitney). Data (n = 3) expressed as mean ± SEM. Stars indicate statistical significance, ns = no significance, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001; c Fluorescent and phase contrast images of pericyte phagocytosis, nuclei (blue), FluoSphere (green). Yellow arrows identify pericytes with phagocytosed beads, and red arrows identify beads that have not been phagocytosed. Scale bar = 150 μm.

Article Snippet: To assess pericyte phagocytosis , FACS-isolated primary human placental pericytes (PromoCell) were cultured in ready-to-use pericyte growth media (PromoCell), seeded at 3.0 × 10 3 cells/cm 2 and allowed to establish in 5% CO 2 in 37 °C humidified air for 48 h before TGF β -1 treatment.

Techniques: Control, Incubation, Flow Cytometry, Fluorescence, MANN-WHITNEY

Journal: Stem Cell Research & Therapy

Article Title: Induced pluripotent stem cell derived pericytes respond to mediators of proliferation and contractility

doi: 10.1186/s13287-024-03671-x

Figure Lengend Snippet: iPericytes are morphologically similar to HBVPs and express pericyte markers. A Phase contrast bright 4 × magnification images of iPSCs, HBVPs, mesoderm iPericytes and neural crest iPericytes. Scale = 200 µm. B-C Fold change gene expression measured by qPCR of pericyte genes PDGFRB, CSPG4, ACTA2 (B) and pluripotency genes OCT4 and NANOG (C) by iPSCs, neural crest iPericytes, mesoderm iPericytes and HBVPs (n = 3 per cell type). Data are normalised to HBVP cells, and comparisons were made using a one-way ANOVA: PDGFRB (F (3, 8) = 103.1, p < 0.0001), CSPG4 (F (3, 8) = 4671, p < 0.0001), ACTA2 (F (3, 8) = 9.340, p < 0.0054), OCT4 (F (3, 8) = 1686, p < 0.0001) and NANOG (F (3, 8) = 606.4, p < 0.0001). Post-hoc comparisons performed using Dunnett’s multiple comparisons test: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Data are shown as mean ± SD. D Immunocytochemistry showing expression of proteins PDGFRβ, CD13, and αSMA (green) by HBVP, mesoderm iPericytes and neural crest iPericytes. Nuclei counter-stained with DAPI (blue). Scale = 10 µm

Article Snippet: Similar results were observed when complete pericyte media (CPM), containing specialised pericyte growth supplement (ScienCell, USA), was used compared to PM (Fig. B).

Techniques: Gene Expression, Immunocytochemistry, Expressing, Staining

Journal: Stem Cell Research & Therapy

Article Title: Induced pluripotent stem cell derived pericytes respond to mediators of proliferation and contractility

doi: 10.1186/s13287-024-03671-x

Figure Lengend Snippet: Mesoderm iPericytes from multiple cell lines have similar mRNA expression. A Principal components analysis showing separate clustering of mesoderm iPericytes and iPSCs from n = 3 different cell lines. B Heat map showing relative expression levels in iPSCs and mesoderm iPericytes of key genes typically expressed by iPSCs, pericytes, endothelial cells (EC), microglia (MG), oligodendrocyte precursor cells (OPCs), oligodendrocytes (OL), astrocytes (AST) and neurons (NEU). Warmer colours indicate higher expression, cooler colours indicate lower expression

Article Snippet: Similar results were observed when complete pericyte media (CPM), containing specialised pericyte growth supplement (ScienCell, USA), was used compared to PM (Fig. B).

Techniques: Expressing

Journal: Stem Cell Research & Therapy

Article Title: Induced pluripotent stem cell derived pericytes respond to mediators of proliferation and contractility

doi: 10.1186/s13287-024-03671-x

Figure Lengend Snippet: Proliferation of iPericytes through the PDGF-BB: PDGFRβ signalling pathway. A iPericytes were incubated in basal pericyte media (PM) and treated with PDGF-BB (PM + PDGF-BB) while being exposed to 100 µM imatinib (PM + PDGF-BB + 100 µM imatinib). Proliferation was measured using an EdU uptake assay. iPericytes that are EdU-positive are indicated by magenta, while total number of iPericytes were measured by DAPI (blue). Scale bar = 50 µm. B Quantification of HBVPs, neural crest iPericytes and mesoderm iPericytes proliferating (as indicated by EdU-positive staining) as a percentage of total cells following 24 h exposure to PM, complete pericyte media with pericyte growth factors (CPM) or PM + PDGF-BB (n = 8 per condition). Data were analysed using a one-way ANOVA: HBVP (F (2, 21) = 35.52, p < 0.0001); neural crest iPericyte (F (2, 21) = 30.85, p < 0.0001); mesoderm iPericyte (F (2, 21) = 191.4, p < 0.0001). C Quantification of changes to PDGF-BB-induced proliferation with increasing concentrations of imatinib over 24 h in HBVPs, neural crest iPericytes and mesoderm iPericytes (n = 8 per condition). Data were analysed using a one-way ANOVA or Kruskal–Wallis test: HBVP (F (3, 26) = 259.2, p < 0.0001); neural crest iPericyte (H (3) = 24.41, p < 0.0001); mesoderm iPericyte (F (3, 28) = 221.5, p < 0.0001). For B , C , post-hoc comparisons were performed using Dunnett’s multiple comparisons or Dunn’s test: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Data shown as mean ± SD. D Heat map of key genes involved in pericyte proliferation in the PDGF-BB: PDGFRβ signalling pathway in HBVP, neural crest iPericytes and mesoderm iPericytes selected from Sweeney et al.

Article Snippet: Similar results were observed when complete pericyte media (CPM), containing specialised pericyte growth supplement (ScienCell, USA), was used compared to PM (Fig. B).

Techniques: Incubation, Staining

Journal: Frontiers in Cell and Developmental Biology

Article Title: Development of a contacting transwell co-culture system for the in vitro propagation of primary central nervous system lymphoma

doi: 10.3389/fcell.2023.1275519

Figure Lengend Snippet: BVPs promote PCNSL cell proliferation in in vitro vitrigel culture. (A) Experimental design for the vitrigel culture assay. PCNSL cells were cultured on vitrigel only (None), in double-sided culture with BVPs (DSC), with BVP-conditioned medium (CM), or with bottom-cultured BVPs (BC). (B) PCNSL cells were cultured under the conditions indicated above for 4 days. The relative cell viability of PCNSL cells was measured after 4 days using CellTiter-Glo. All graphs are presented as mean ± SD ( n = 3), Welch’s t -test (two-tailed), ** p < 0.01, *** p < 0.001. (C) Vitrigel culture assay of various human epithelial cells. PCNSL cells were co-cultured with pericytes, astrocytes, HUVECs, or fibroblasts. After 4 days, cell viability was measured by CellTiter-Glo. All graphs are presented as mean ± SD ( n = 3), Welch’s t -test (two-tailed), * p < 0.05. (D) PCNSL cells were cultured with or without BVPs and harvested at the indicated time points. Relative cell number was measured using CellTiter-Glo. All graphs are presented as mean ± SD ( n = 3), Welch’s t -test (two-tailed), *** p < 0.001.

Article Snippet: Human BVPs were purchased from Angio-Proteomie (Boston, USA) and cultured in Pericyte Media (PromoCell, Heidelberg, Germany).

Techniques: In Vitro, Cell Culture, Two Tailed Test

Journal: Frontiers in Molecular Neuroscience

Article Title: The impaired distribution of adenosine deaminase isoenzymes in multiple sclerosis plasma and cerebrospinal fluid

doi: 10.3389/fnmol.2022.998023

Figure Lengend Snippet: The activity of both cell-surface ecto-adenosine deaminase (eADA) iso-enzymes was highest in the brain microvascular endothelial cells. Representative images of the human brain astrocytes (HASTR), human vascular pericytes (HBVP) and human brain microvascular endothelial cells (HBMECs), scale bar = 50 μm (A) . The activities of cell-surface total eADA (B) , eADA1 (C) and eADA2 (D) in HASTR, HBVP and HBMEC (B) . Results are shown as mean ± SEM, N = 3 independent experiments; n = 6 biological replicates per experiment; *** p < 0.001, **** p < 0.0001 by One-way ANOVA followed by Kruskal-Wallis post-hoc test ( n = B–D ); ns, not significant.

Article Snippet: Pericytes were grown in the pericytes growth media (ScienCell, #1201) supplemented with 4 μg/ml blasticidin S as described by .

Techniques: Activity Assay

Journal: Frontiers in Molecular Neuroscience

Article Title: The impaired distribution of adenosine deaminase isoenzymes in multiple sclerosis plasma and cerebrospinal fluid

doi: 10.3389/fnmol.2022.998023

Figure Lengend Snippet: Treatment of HBVP with IL17 and TNFα did not affect cell-surface ecto-adenosine deaminase (eADA) activity. Representative images of human brain vascular pericytes treated for 18 h with 50 ng/ml IL17 and 10 ng/ml TNFα, scale bar = 50 μm (A) . Representative chromatogram with signals for adenosine triphosphate (ATP), adenosine diphosphate (ADP), and nicotinamide adenine dinucleotide (NAD) in control (black) and IL17/TNFα-treated (pink) pericytes (B) . Intracellular ATP (C) and ADP (D) concentration and ATP/ADP ratio (E) , NAD (F) concentration and ATP/NAD ratio (G) in IL17/TNFα-treated pericytes. The rate of cell-surface ATP (H) and AMP (I) hydrolysis and the activity of cell-surface total eADA (J) , eADA1 (K) and eADA2 (L) in IL17/TNFα-treated pericytes. Results are shown as mean ± SEM; N = 3 independent experiments; n = 2 (C–G) , n = 6 (H–L) biological replicates per experiment; ** p < 0.01; **** p < 0.0001 by Student t -test (C,D) and Mann-Whitney U -test (E–G) ns, not significant.

Article Snippet: Pericytes were grown in the pericytes growth media (ScienCell, #1201) supplemented with 4 μg/ml blasticidin S as described by .

Techniques: Activity Assay, Control, Concentration Assay, MANN-WHITNEY